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nlrc4 inflammasome activator flagellin  (InvivoGen)


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    Structured Review

    InvivoGen nlrc4 inflammasome activator flagellin
    Nlrc4 Inflammasome Activator Flagellin, supplied by InvivoGen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nlrc4+inflammasome+activation/pm37153555-64-62-68
    Average 86 stars, based on 1 article reviews
    nlrc4 inflammasome activator flagellin - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Activation Assay:

    Article Title: Herpes simplex virus 1 harboring poly(T) DNA sequences as a key ligand for AIM2 inflammasome activation and host defense.
    Article Snippet: AR TI CL E IN P RE SS Virus culture, infection, and cell stimulations Human HSV-1 HF strain (ATCC; VR-260), HSV-1 F strain (ATCC; VR-733), and HSV-1 KOS strain (ATCC; VR-1493) were purchased and propagated in Vero cells (gift from Dr. Atsushi Kawaguchi, University of Tsukuba); the virus titer was measured using the plaque assay in Vero cells. .. For ligandmediated AIM2 inflammasome activation, 1 ng of poly(dA:dT) (InvivoGen, tlrl-patn), poly(dG:dC) (InvivoGen, tlrl-pgcn), or poly(T) DNA of 10-, 30-, or 90-mer length was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. For NLRC4 inflammasome activation, 10 ng of flagellin (Invivogen, tlrl-epstfla5) was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. In the context of VP22, F strain VP22 vector (pLV[Exp]-EGFP:T2A:Puro-EF1A>VP22) was purchased (Vector Builder, VB2209201383rfw) and BMDMs were transfected with the VP22 expression plasmid 12 h prior to viral infection using DOTAP (Roche, 11202375001). .. For HSV-1 (16 h; MOI 10) infection, primary BMDMs and iBMDMs were cultured in DMEM high glucose (Gibco, 11995-065).

    Transfection:

    Article Title: Herpes simplex virus 1 harboring poly(T) DNA sequences as a key ligand for AIM2 inflammasome activation and host defense.
    Article Snippet: AR TI CL E IN P RE SS Virus culture, infection, and cell stimulations Human HSV-1 HF strain (ATCC; VR-260), HSV-1 F strain (ATCC; VR-733), and HSV-1 KOS strain (ATCC; VR-1493) were purchased and propagated in Vero cells (gift from Dr. Atsushi Kawaguchi, University of Tsukuba); the virus titer was measured using the plaque assay in Vero cells. .. For ligandmediated AIM2 inflammasome activation, 1 ng of poly(dA:dT) (InvivoGen, tlrl-patn), poly(dG:dC) (InvivoGen, tlrl-pgcn), or poly(T) DNA of 10-, 30-, or 90-mer length was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. For NLRC4 inflammasome activation, 10 ng of flagellin (Invivogen, tlrl-epstfla5) was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. In the context of VP22, F strain VP22 vector (pLV[Exp]-EGFP:T2A:Puro-EF1A>VP22) was purchased (Vector Builder, VB2209201383rfw) and BMDMs were transfected with the VP22 expression plasmid 12 h prior to viral infection using DOTAP (Roche, 11202375001). .. For HSV-1 (16 h; MOI 10) infection, primary BMDMs and iBMDMs were cultured in DMEM high glucose (Gibco, 11995-065).

    Incubation:

    Article Title: Herpes simplex virus 1 harboring poly(T) DNA sequences as a key ligand for AIM2 inflammasome activation and host defense.
    Article Snippet: AR TI CL E IN P RE SS Virus culture, infection, and cell stimulations Human HSV-1 HF strain (ATCC; VR-260), HSV-1 F strain (ATCC; VR-733), and HSV-1 KOS strain (ATCC; VR-1493) were purchased and propagated in Vero cells (gift from Dr. Atsushi Kawaguchi, University of Tsukuba); the virus titer was measured using the plaque assay in Vero cells. .. For ligandmediated AIM2 inflammasome activation, 1 ng of poly(dA:dT) (InvivoGen, tlrl-patn), poly(dG:dC) (InvivoGen, tlrl-pgcn), or poly(T) DNA of 10-, 30-, or 90-mer length was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. For NLRC4 inflammasome activation, 10 ng of flagellin (Invivogen, tlrl-epstfla5) was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. In the context of VP22, F strain VP22 vector (pLV[Exp]-EGFP:T2A:Puro-EF1A>VP22) was purchased (Vector Builder, VB2209201383rfw) and BMDMs were transfected with the VP22 expression plasmid 12 h prior to viral infection using DOTAP (Roche, 11202375001). .. For HSV-1 (16 h; MOI 10) infection, primary BMDMs and iBMDMs were cultured in DMEM high glucose (Gibco, 11995-065).

    Expressing:

    Article Title: Herpes simplex virus 1 harboring poly(T) DNA sequences as a key ligand for AIM2 inflammasome activation and host defense.
    Article Snippet: AR TI CL E IN P RE SS Virus culture, infection, and cell stimulations Human HSV-1 HF strain (ATCC; VR-260), HSV-1 F strain (ATCC; VR-733), and HSV-1 KOS strain (ATCC; VR-1493) were purchased and propagated in Vero cells (gift from Dr. Atsushi Kawaguchi, University of Tsukuba); the virus titer was measured using the plaque assay in Vero cells. .. For ligandmediated AIM2 inflammasome activation, 1 ng of poly(dA:dT) (InvivoGen, tlrl-patn), poly(dG:dC) (InvivoGen, tlrl-pgcn), or poly(T) DNA of 10-, 30-, or 90-mer length was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. For NLRC4 inflammasome activation, 10 ng of flagellin (Invivogen, tlrl-epstfla5) was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. In the context of VP22, F strain VP22 vector (pLV[Exp]-EGFP:T2A:Puro-EF1A>VP22) was purchased (Vector Builder, VB2209201383rfw) and BMDMs were transfected with the VP22 expression plasmid 12 h prior to viral infection using DOTAP (Roche, 11202375001). .. For HSV-1 (16 h; MOI 10) infection, primary BMDMs and iBMDMs were cultured in DMEM high glucose (Gibco, 11995-065).

    Plasmid Preparation:

    Article Title: Herpes simplex virus 1 harboring poly(T) DNA sequences as a key ligand for AIM2 inflammasome activation and host defense.
    Article Snippet: AR TI CL E IN P RE SS Virus culture, infection, and cell stimulations Human HSV-1 HF strain (ATCC; VR-260), HSV-1 F strain (ATCC; VR-733), and HSV-1 KOS strain (ATCC; VR-1493) were purchased and propagated in Vero cells (gift from Dr. Atsushi Kawaguchi, University of Tsukuba); the virus titer was measured using the plaque assay in Vero cells. .. For ligandmediated AIM2 inflammasome activation, 1 ng of poly(dA:dT) (InvivoGen, tlrl-patn), poly(dG:dC) (InvivoGen, tlrl-pgcn), or poly(T) DNA of 10-, 30-, or 90-mer length was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. For NLRC4 inflammasome activation, 10 ng of flagellin (Invivogen, tlrl-epstfla5) was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. In the context of VP22, F strain VP22 vector (pLV[Exp]-EGFP:T2A:Puro-EF1A>VP22) was purchased (Vector Builder, VB2209201383rfw) and BMDMs were transfected with the VP22 expression plasmid 12 h prior to viral infection using DOTAP (Roche, 11202375001). .. For HSV-1 (16 h; MOI 10) infection, primary BMDMs and iBMDMs were cultured in DMEM high glucose (Gibco, 11995-065).

    Infection:

    Article Title: Herpes simplex virus 1 harboring poly(T) DNA sequences as a key ligand for AIM2 inflammasome activation and host defense.
    Article Snippet: AR TI CL E IN P RE SS Virus culture, infection, and cell stimulations Human HSV-1 HF strain (ATCC; VR-260), HSV-1 F strain (ATCC; VR-733), and HSV-1 KOS strain (ATCC; VR-1493) were purchased and propagated in Vero cells (gift from Dr. Atsushi Kawaguchi, University of Tsukuba); the virus titer was measured using the plaque assay in Vero cells. .. For ligandmediated AIM2 inflammasome activation, 1 ng of poly(dA:dT) (InvivoGen, tlrl-patn), poly(dG:dC) (InvivoGen, tlrl-pgcn), or poly(T) DNA of 10-, 30-, or 90-mer length was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. For NLRC4 inflammasome activation, 10 ng of flagellin (Invivogen, tlrl-epstfla5) was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. In the context of VP22, F strain VP22 vector (pLV[Exp]-EGFP:T2A:Puro-EF1A>VP22) was purchased (Vector Builder, VB2209201383rfw) and BMDMs were transfected with the VP22 expression plasmid 12 h prior to viral infection using DOTAP (Roche, 11202375001). .. For HSV-1 (16 h; MOI 10) infection, primary BMDMs and iBMDMs were cultured in DMEM high glucose (Gibco, 11995-065).



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